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CRISPR mutagenesis introduced a 1644bp deletion from the middle of exon 3 to the middle of exon 4, resulting in a frame-shift with a deletion of 248 nucleotides in the mRNA product and the introduction of a premature stop codon. Western blot analysis of tissue lysates from embryos showed that the encoded protein is absent in the homozygous mutant samples. (J:242905)