通过与CAG-Cre小鼠杂交,去除了LoxP引导的exon 1。使用兔抗Lrrc6多克隆抗体对气管进行 Western blot 分析,证实了纯合突变小鼠中无蛋白表达(来源:J:258369)。
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LoxP-flanked exon 1 was removed by crossing with CAG-Cre mice. Western blot analysis of the trachea using a rabbit anti-Lrrc6 polyclonal antibody confirmed the absence of protein expression in homozygous mutant mice. (J:258369)