Adamts16^em2(IMPC)H

This allele from IMPC was generated at Medical Research Council Harwell by injecting CAS9 RNA and 4 guide sequences which resulted in a 906nt deletion. The allele results in a complete loss of exon 5, which encodes part of the highly conserved, zinc-dependent metalloprotease domain; splicing from exon 4 to exon 6 results in a predicted frame-shift and early chain termination due to a premature stop codon. qRT-PCR analysis showed a severe reduction of expression in homozygous mutant kidneys. (J:237616, J:283272)