Tg(Aldh1l1-cre/ERT2)02Kan

The transgene was generated by inserting a cre/ERT2 cassette into intron 1/ exon 2 of the mouse Aldh1l1 in a murine BAC (BAC RP23-7M9) by homologous recombination in bacteria. Pronucleus injection gave rise to 5 transgenic founder mice. Three out of five founder mice that were crossed with reporter mice showed expression in the brain. Only one line (line 02) showed robust expression in forebrain astrocytic cells and minimal expression in other cell types of the brain and was used for further analyses. (J:247456)