在第2外显子上游插入了loxP位点和FRT引导的pgk-puro元件,通过同源重组在第4外显子下游也插入了loxP位点。通过Cre介导的重组,移除了pgk-puro元件和2-4号外显子。RT-PCR和 Western blot 实验在8周龄纯合小鼠的睾丸中证实了表达缺失。(来源:J:251285)
Basic Information
(C57BL/6J x 129S6/SvEvTac)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count