在ES细胞中,通过同源重组插入了带有tdTomato/T2A/Cre-ERT2/bGHpolyA的磁带,其起始密码子与小鼠Mex3a的exon1对应。筛选出阳性克隆后,这些克隆经FlpO质粒转染以去除neo cassette。阳性克隆被选中,然后进行囊胚注射以产生嵌合体。成功传代至生殖系统的嵌合体与C57BL6小鼠杂交,产生后代用于最终的鼠群繁育。(来源:J:253826)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count