在ES细胞中,通过同源重组插入了带有tdTomato/T2A/Cre-ERT2/bGHpolyA的磁带,其起始密码子与小鼠Mex3a的exon1对应。筛选出阳性克隆后,这些克隆经FlpO质粒转染以去除neo cassette。阳性克隆被选中,然后进行囊胚注射以产生嵌合体。成功传代至生殖系统的嵌合体与C57BL6小鼠杂交,产生后代用于最终的鼠群繁育。(来源:J:253826)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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