A loxP site and an FRT-flanked phosphoglycerate kinase-neomycin (PGK-Neo) selection cassette were inserted upstream of exon 3 and a second loxP site downstream of exon 3. Cre-mediated recombination removed the PGK-Neo and exon 3. Western blot analysis confirmed absence of protein in MEFs and bone marrow derived macrophages. (J:257411)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count