Gt(ROSA)26Sor^{tm1(tTA,tetO-APOE_i3,-EGFP)Gbu}

A construct consisting of a floxed neo cassette, tTA site, a CMV promoter with a tetracycline responsive element, a human APOE_i3 cDNA, an IRES site and an EGFP gene, was inserted via homologous recombination. In the absence of Cre-mediated recombination the human protein is not expressed. Following Cre-mediated recombination the human protein is expressed and this expression may be suppressed with the addition of doxycycline. (J:256115)