Gja1^tm2Clo

Three amino acid substitutions, an alanine for serine at amino acid 330 (TCC to GCA), a tyrosine for serine at amino acid 328 (TCC to TAC), and an alanine for serine at amino acid 325 (AGC to GCA), at sites phosphorylated by casein kinase 1, were introduced into exon II and a floxed PGK-Neo-Stop cassette was inserted upstream of exon II. Western blot analysis of whole heart lysates showed that the protein expression level is reduced by nearly 30%. (J:253159)