CRISPR/Cas9导向的基因组编辑技术用于在第3内含子插入反向LoxP位点,以及带有FRT标记的PGK-neomycin抗性元件。同时,反向LoxP位点被放置在第4内含子。通过Flp介导的重组,FRT标记的neo cassette被移除,导致第4外显子被敲除。(来源:J:257337)
Basic Information
(C57BL/6 x 129S6/SvEvTac)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count