CRISPR/Cas9导向的基因组编辑技术用于在第3内含子插入反向LoxP位点,以及带有FRT标记的PGK-neomycin抗性元件。同时,反向LoxP位点被放置在第4内含子。通过Flp介导的重组,FRT标记的neo cassette被移除,导致第4外显子被敲除。(来源:J:257337)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S6/SvEvTac)F1
Endonuclease-mediated
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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