Exon 2被用同源重组的方式替换为一个带有floxed MC1-neo cassette,通过 Western blot 实验确认了纯合小鼠中没有蛋白表达(来源:J:256656)
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Exon 2 was replaced with a floxed MC1-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in homozygous mice. (J:256656)