An FRT site, IRES-beta-gal, loxP site, neo cassette, FRT site and loxP site were inserted in intron 1 and a loxP site was inserted in intron 3 via homologous recombination using the EUCOMM targeting vector HTGR06009_A_2_G03. Flp mediated recombination removed the beta-gal and neo cassettes. Cre mediated recombination removed exons 2 and 3. (J:256610)