Ucp1^m1Hhz

A spontaneous point mutation resulted in a C-to-A substitution at position 39 in exon 5. This mutation produces an alternative CAG splice acceptor site from CCG, the use of which results in the deletion of the first 39 nucleotides of exon 5 and 13 amino acids from the encoded peptide. Western blot analysis confirmed the absence of protein expression in brown adipose tissue from homozygous mice. (J:256980)