Atp4a^tm1.1Ocalv

Exon 14 was replaced with a modified exon 14 in with CGA was mutated to TGC at position 709 resulting in substitution of cysteine to arginine and a silent mutation in which CTG was mutated to CTT in codon 709 (to generate a HindIII site). A FRT-flanked PGK/bg2-neo resistance cassette was introduced into the intron preceding 5' to exon 13, with a transcriptional orientation opposite to that of the gene. Flp-mediated recombination removed the selection cassette. This mutation corresponds to the human p.R703C mutation responsible for atypical type I familial gastric neuroendocrine tumors. (J:235360)