Tg(Foxp3*-cre,-Thy1)#Xzhou

Transgene consists of the hCre-2A-eqFP650-2A-Thy1.1 cDNA fragment inserted immediately downstream of the Foxp3 ATG translational start site by homologous recombination into a 188-kb mouse BAC (from the C57BL/6 genome; clone RP23-143D8) carrying the intact Foxp3 gene was used. The CNS1 enhancer in Foxp3 gene was deleted by another round of homologous recombination. The modified BAC was purified and microinjected into the pronuclei of a (B6xB6D2) F1 mouse fertilized ovum to generate transgenic line of mice. Three founder mice were generated and screened for Thy1.1 and endogenous Foxp3 expression.The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:252233)