同源重组被用来将大部分编码区域(从exon 2到exon 4)替换为β-半乳糖苷酶报告基因和诺卡因选择标记。 Western blot确认了纯合子中无蛋白表达。(来源:J:242841)
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Homologous recombination was used to replace most of the coding region (exon 2 to exon 4) with a beta-galactosidase reporter and a neomycin selection cassette. Western blot confirmed absence of protein in homozygotes. (J:242841)