Penk^{tm1.1(cre)Gsche}

The targeting vector was constructed using recombineering techniques. An 8,528 bp genomic DNA fragment containing the last exon of the mouse Penk gene was retrieved from BAC clone RP23-RP23- 365K8 to a vector containing the DT gene. A cassette of IRES-Cre frt-PGKNeo-frt was inserted between stop codon TGA and 3' UTR. The neo cassette was removed by breeding germline chimeras with FLP1 expressing homozygous females. (J:253245)