通过sgRNA和CRISPR/Cas9技术,一个FLAG序列被插入到了编码序列的3'末端,紧接在终止密码子的上游。sgRNA和Cas9表达的质粒,以及参照质粒,被转染到了EGR-G01 ES细胞中。随后,这些ES细胞被注入到8细胞的ICR胚胎中。(来源:J:243766)
Basic Information
(129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count