Igs2^tm2Kng

The targeting construct contains an attB site, mouse Hsp68 minimal promoter, a human regulatory region (H2), mouse Kitl cDNA, SV40, an attB site and FRT5. The construct and phiC31 mRNA were co-injected into a single pronucleus of FVB-H11P3 zygotes. H2 is regulatory enhancer located in an intergenic region 350 kb upstream of the Kitl transcription start site, it contains a non-coding SNP (rs12821256) associated with hair color. The ancestral (ANC) polymorphism (A) is associated with brown hair, and the blond (BLD) polymorphism (G) is associated with blond hair. This allele carries the G polymorphism. (J:248381)