在同源重组过程中,第3外显子被替换成lacZ基因和一个floxed pgk-neo元件。随后,通过Cre介导的重组移除了neo cassette。 Western blot结果显示,纯合小鼠中蛋白质表达确实缺失了(来源:J:229548)
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Exon 3 was replaced with a lacZ gene and a floxed pgk-neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. Western blot analysis confirmed loss of protein expression in homozygous mice. (J:229548)