Tg(CAG-PPARG,-tdTomato)#Sig

The transgenic construct comprised the following elements: a CAG promoter, a loxP site flanked STOP cassette, a splicable intron, the human PPARG coding region (from a cDNA), an IRES in front of the tdTomato reporter gene, a WPRE transcription enhancer and a polyA signal. The construct was injected into the pronuclei of fertilized C57BL/6 x SJL eggs. Only after cre-mediated recombination (which will remove the STOP cassette), will the human and reporter genes be expressed. (J:242422)