Exon 2被插入了一个IRES-lacZ-neo插件,导致其功能受损。 Western blot结果显示,纯合突变胚胎提取物中PKN1蛋白表达缺失,且PKN2或PKN3的表达没有相应补偿性改变。(来源:J:229595)
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Exon 2 was disrupted with the insertion of an IRES-lacZ-neo cassette. Western blot analysis confirmed the absence of PKN1 expression in homozygous mutant embryo extracts with no compensatory changes in PKN2 or PKN3 expression. (J:229595)