Tg(Ucp1-icre)#Jcbr

A cassette containing iCre recombinase, an artificial intron, a bovine growth hormone polyadenylation signal, and an ampicillin-resistance gene flanked by FRT sites (Flpe recombinase recognition sequence) was recombined into the first exon of the Ucp1 gene of a bacterial artificial chromosome (BAC RP23-248O16). An FRT site flanked ampicilin-resistance gene and a loxP site were removed. DNA from the modified BAC clone was used as the source for the transgene, which was microinjected into the pronucleus of C57BL/6N fertilized eggs. One of four founders expressed the transgene, and was selected for analysis. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:215519)