Tg(Gpr101-cre)BKldr

A construct (composed of cre recombinase with a nuclear localization signal, SV40 polyadenylation sequence, and an Frt-Kanamycin/neo-Frt cassette) was introduced into the ATG site in exon 2 of the Gpr101 gene in a bacterial artificial chromosome (BAC RP23-203E19) by recombineering in bacterial cells. Modified BAC DNA was purified and injected into mouse pronuclei to produce transgenic mice. Two lines A and B are generated and analized separately. (J:242614)