Tg(ACTB-Wld*)3Cole

A construct consisting of the human beta actin promoter followed by a mutant cDNA for Wlds that contains engineered C>G point mutations changing arginine codons 213 and 215 to alanine (p.Arg213Ala, p.Arg215Ala), was injected into the pronuclei of fertilized (C57BL/6 x CBA)F1 eggs that do not express Wlds. The two mutations weaken or disable the nuclear localization signal, thus changing the localization of the translated peptide towards the cytoplasm. A small percentage of peptides is still directed to the nucleus. (J:144846)