包含Npr2基因cDNA(编码8-22号外显子,以及IRE斯序列、eGFP基因和FRT引导的pgk-neoCas9 cassette)的敲除构建被插入到第7号内含子中,通过同源重组在8和9号外显子的7个苏氨酸和Thr位点引入了点突变。这些点突变将苏氨酸和Thr的密码子替换为谷氨酸密码子。通过Cre介导的重组,整个构建被去除。产生的蛋白质表现得像是持续磷酸化状态。(来源:J:228790)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129 x C57BL/6J)F1
Targeted
Insertion, Nucleotide substitutions
--
1
3
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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