Kat14^{tm1d(KOMP)Wtsi}

The L1L2_gt1 cassette was inserted at position 144217323 of Chromosome 2 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon at position 144218113. The critical exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:236024)