全长小鼠cDNA被克隆到pCDNA3.1(+)载体上,载体的CMV启动子下游,以及牛生长激素(BGH)的polyadenylation信号和转录终止序列上游。经过酶切的载体被注入C57BL/6J受精卵的核中。建立了5个转基因供体,编号为#14, #50, #55, #63和#79。供体#50的F1、F2和F3代中,转基因拷贝数平均约为17。 Western blot结果显示,检测到了转基因产生的18 kDa蛋白表达。(来源:J:243722)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6J
--
插入
--
1
--
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部