Nlrp3^{tm2.1(NLRP3)Bhk}

A Replacer vector containing a mutated loxP site and the human ortholog of the mouse gene was injected into Nlrp3tm1Bhk ES cells. In these ES cells the endogenous locus was replaced with an FRT site flanked neomycin resistance gene cassette which was then removed through flp-mediated recombination. The cells also contain the same mutated loxP site as the Replacer vector. The injected ES cells were subjected to cre-mediated recombination to integrate the vector into the genome. The resulting allele corrects the null allele of the ES cells by inserting the human ortholog into the location of the endogenous gene. The inserted gene is flanked by an upstream mutated loxP site and a downstream FRT site. Expression of the inserted sequence was confirmed by qPCR. (J:241705)