通过胚胎干细胞中同源重组,构建了一个用Venus-Cre和FRT介导的PGK-Neo cassette替换内源性Bhlha9基因的向量。重组后,虽然通过Southern blotting和PCR确认了基因组变异,但肢体芽的Venus-Cre蛋白表达并未检测到(来源:J:243551)
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count