通过胚胎干细胞中同源重组,构建了一个用Venus-Cre和FRT介导的PGK-Neo cassette替换内源性Bhlha9基因的向量。重组后,虽然通过Southern blotting和PCR确认了基因组变异,但肢体芽的Venus-Cre蛋白表达并未检测到(来源:J:243551)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
插入,基因内删除
--
1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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