Stag2^{tm1c(EUCOMM)Wtsi}

The L1L2_gt0 cassette was inserted upstream of the critical exon (exon 7) with insertion at bp position ChrX:42,222,777 [Build GRCm38]. The L1L2_gt0 cassette is composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 7 at bp position ChrX:42,223,727 [Build GRCm38]; therefore flanking exon 7 with loxP sites. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 7 floxed. (J:173534)