Sirt1^tm2.1Arte

Two substitutions (AC>GA) were engineered to change codon 522 in exon 8 from threonine to glutamine (p.Thre522Glu). Glutamine is a phosphomimetic, simulating a phosphorylated threonine and rendering the protein constitutively active. A loxP site and an FRT site flanked selectable marker cassette were inserted into intron 7. The marker cassette was removed through subsequent flp-mediated recombination. (J:241351)