在ES细胞中,通过同源重组技术,将一个icre/ERT2 cassette紧接着一个Frt-flanked neo selection cassette插入到了起始密码子ATG之后的顺位。经过定向筛选的正确克隆ES细胞被注入C57BL/6的囊胚细胞。随后,这些小鼠与表达Flp的实验性小鼠杂交,以删除neo cassette。(来源:J:201085)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
12
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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