Tnfaip3^tm3.1Vmd

A tyrosine to alanine mutation and a phenylalanine to alanine mutation were inserted at position 599 and 600 (Y599A and F600A; TATTTT to GCTGCA) in exon 7 and an FRT flanked neo cassette was inserted at position chr10:18724150 (NCBI37/mm9) via homologous recombination. Flp mediated recombination removed the neo cassette. The mutations compromise ZnF4 ubiquitin binding. (J:228302)