Orai2^{tm1.1(KOMP)Vlcg}

The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 11345bp between positions 136626234-136637578 of Chromosome 5 (Genome Build37), deleting virtually the entire coding region (located on exons 2 and 3). The cassette contains a lacZ reporter gene cassette and a loxP site flanked neomycin resistance gene cassette. The neo gene was removed through subsequent cre-mediated recombination. The absence of transcription and translation from this allele was confirmed by qRT-PCR and immunoblots. (J:241365)