在exon 1上游插入了一个由loxP位点引导的诺卡因抗性基因座,其中包含了一个15氨基酸的Avi标签,使其在基因翻译时带有N端的生物素化标记。插入诺卡因座是为了干扰该基因的转录,通过RT-qPCR实验确认,确实将表达量降低到野生型的约27%。(来源:J:240473)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入
--
1
10
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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