Log In|Sign Up
中文
这条在Gt(ROSA26)Sor定位的敲入线包含了以下遗传元件，按5'至3'的顺序排列：CAG启动子、loxP-3xpolyadenylation site-loxP(“lox-stop-lox”)、附着于膜上的(通过N端myristoylation)修改过的tdTomato，带有C端的三my标签、2A切割位点、H2B-GFP融合蛋白，带有C端的三抗原标签、牛生长激素基因3'UTR以及Frt-PGK-Neo-Frt。在没有Cre介导的重组情况下，tdTomato和GFP报告基因均不表达。当与表达Cre重组酶的转基因或敲入品系杂交时，表达Cre的细胞中会剔除lox-stop-lox序列，产生膜结合的tdTomato和核内的H2B-GFP。这些荧光蛋白可以通过它们的内在荧光或通过免疫标记荧光蛋白及其关联的表位标签来观察。来源：(J:269489)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
