将含小鼠Col2a1启动子/增强子驱动的d2EGFP表达的构建,注入到C57BL/6 x SJL F2胚胎中。d2EGFP来源于pd2EGFP-N1质粒,其通过将小鼠 ornithine decarboxylase (MODC) 的422-461位点与EGFP的C端连接,产生了一种改良的、非稳定且红光位移的野生型GFP变体。这种修改是为了在哺乳动物细胞中获得更强的荧光和更高表达量(来源:J:169056)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x SJL)F2
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2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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