App^tm1.1Wevn

The targeting vector was designed to substitute an arginine for isoleucine at amino acid position 13 by introducing two point mutations into the arginine codon (CGC to ATC). A loxP-flanked Neomycin cassette also was included in the construct. The R13I mutation is located on a 10 kb long arm fragment 300 bp downstream of exon 7. Cre-mediated recombination removed the loxP-flanked neo. Expression of APP protein is not affected. (J:241632)