靶向载体被用来在内含子1插入一个loxP位点和一个由FRT序列包裹的PGK-NEO元件,随后是exon 3下游的第二个loxP位点。通过Flp介导的重组,FRT标记的neo元件被移除,剩下exons 2和3被标记为可剪接。随后,Cre介导的重组去除exons 2和3。(来源:J:229949)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6J x 129S4/SvJae)F1
Targeted
基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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