靶向载体被用来在内含子1插入一个loxP位点和一个由FRT序列包裹的PGK-NEO元件,随后是exon 3下游的第二个loxP位点。通过Flp介导的重组,FRT标记的neo元件被移除,剩下exons 2和3被标记为可剪接。随后,Cre介导的重组去除exons 2和3。(来源:J:229949)
Basic Information
(C57BL/6J x 129S4/SvJae)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count