Sdc4^em#Qin

The first exon of the gene was targeted using the CRISPR/Cas9 system using two sgRNAs (5'-GCAGCAGCAGCGGCGCAAGC-3' and 5'-AGCATCTTCGTCGTCGGGGA-3'). This resulted in three mouse lines with different mutations: 1 bp deletion, 10 bp deletion and 1 bp insertion. All three mutations cause reading frameshifts and premature translation termination. Immunoblots confirmed absence of peptides produced from this allele. It is not specified whether a pool of the three mouse lines was used or a specific single line. (J:239894)