Asic3^tm2.1Cibms

The sequence coding for exon 1, intron 1 and most of exon 2 was replaced with the following: an EGFP reporter gene cassette, an FRT site flanked neomycin resistance gene cassette and the WGA (wheat germ agglutinin) cDNA. LoxP sites were placed upstream of the EGFP cassette and between the neo cassette and the WGA cDNA. The neo cassette was removed through subsequent flp-mediated recombination. RT-qPCR showed barely detectable levels of target gene transcripts in mice homozygous for the allele and abundant expression of the EGFP knock-in gene. (J:239934)