在前导序列上游插入了loxP位点,接着在内含子1中插入了FRT表型开关位点和诺卡因抗性基因座,再配对一个loxP位点。通过RT-PCR分析,发现诺卡因基因座干扰了该等位基因的转录。随后通过flp介导的重组去除了诺卡因基因座。接着,通过cre介导的重组删除了exon 1,形成了无功能的等位基因。RT-PCR确认了这个等位基因转录本的减少。(来源:J:239934)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入,基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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