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A CAG promoter and a loxP site flanked puromycin resistance gene and ECFP reporter gene double cassette were inserted upstream of exon 1 replacing the native promoter. Subsequent cre-mediated recombination removed the selection/reporter cassette, bringing the gene under the constitutively active control of the CAG promoter. The expression from this allele is around tenfold higher than from the wild-type allele. The half-life of the transcripts is the same as wild-type. (J:239949)