在exon 5的3'端插入了一个单个的loxP位点。接着,将Frt-PGK-Neo-Frt-loxP片段插入到exon 3的5'端。通过与β-actin启动子驱动Frt重组酶的鼠杂交,移除了neo cassette,使得exons 3-5两侧各有一个loxP位点。(来源:J:237513)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count