Foxc2^{tm1.1(cre/ERT2)Rssr}

A targeting vector with a cDNA sequence encoding the CreER fusion gene, followed by a frt-flanked neomycin selection cassette replaced the open reading frame of the Foxc2 gene via homologouse recombination in C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6 for germline transmission. At some point, they were bred with ROSA26::FLPe mice (on a C57BL/6J genetic background) for germline deletion of the neo selection cassette. (J:228882)