Bcl11a^em2Sho

The dominant +62 DNase I Hypersensitive Site in a 10kb enhancer region in intron 2 was targeted for deletion with the CRISPR/Cas9 system. In human, mutations in the orthologous enhancer region are associated with various hemoglobin disorders. The gRNA sequences used were 5'-TCGCTGCCTTCAGTTCTGCT-3' and 5'-CTATTTCCATTGGTGGATAC-3'. (J:239331, J:260023)