在内皮1插入了一个带有诺卡因抗性基因座的FRT位点。外显子1通过两个密码子改变进行了靶向:L54由CTT变为CTG的silent突变,以及S55A,由TCA变为GCC,这产生了一个MscI切割位点,同时将丝氨酸55替换为 Alanine,阻止了该位置肽的磷酸化。(来源:J:238996)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Targeted
Insertion, Nucleotide substitutions
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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