一个敲除的诺卡因抗性基因座替换了1到3号外显子。通过Cre介导的重组,去除了选择性基因座。 Western blot结果显示长骨中没有蛋白表达(来源:J:237104)
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A floxed neomycin resistance cassette replaced exons 1 through 3. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in the long bones. (J:237104)