Prdx6^tm3.1Abf

The targeting vector is designed to insert, by site-directed mutagenesis, a TGC to TCC point mutation followed by a FRT-flanked neomycin cassette in exon 2. The mutation results in an alteration of the corresponding amino acid from cysteine to serine (C47S) at codon 47. Flp-mediated recombination removed the FRT-flanked neo cassette. The mutation does not affect phospholipase A2 activity, but does eliminate peroxidase activity. (J:239138)